The effect of high-fat diet and exercise on KISS-1/GPR54 expression in testis of growing rats

Abstract Purpose To find the expression of KISS-1 and G protein-coupled receptor 54 in rats testis from PND 21st to 56th. Method 128 three-week-old weaned underwent high-fat diet exercise (60–70% VO 2max , 1 h/day, 5 days/week) intervention were randomly divided into group C, CE, HC, or HE. Sample time points set on 21st, 35th, 43rd, The testicular testosterone mRNA content, protein content GPR54 tissue detected by ELISA, RT-qPCR, Western blotting. Result (1) increased gradually during growing period. peaked at 35D 43D. (2) High-fat affected KISS-1/GPR54 system rat reduced level protein. (3) 60–70% 2 max decreased level. Exercise improved development with a diet. Conclusion can downregulate gene change trend. KISS-1/GPR54, which may be involved effects dietary sex hormone disorders.